Composite

Part:BBa_K4343066:Design

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)


pUC-HUH-EgElo9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 5470
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1920
    Illegal XhoI site found at 1953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2108
    Illegal AgeI site found at 97
    Illegal AgeI site found at 458
    Illegal AgeI site found at 3161
    Illegal AgeI site found at 3522
    Illegal AgeI site found at 4664
    Illegal AgeI site found at 5673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 638
    Illegal BsaI site found at 3702
    Illegal BsaI site found at 4239
    Illegal BsaI site found at 4667
    Illegal BsaI site found at 5194
    Illegal BsaI site found at 5676
    Illegal BsaI.rc site found at 4661
    Illegal BsaI.rc site found at 4723
    Illegal BsaI.rc site found at 5670
    Illegal BsaI.rc site found at 5940
    Illegal SapI.rc site found at 363
    Illegal SapI.rc site found at 3427


Design Notes

NONE


Source

The EgElo9 gene from Euglena gracilis.

References